MoRitS is a cooperation of

University of Bielefeld

University of Applied Sciences Bielefeld

government-funded by

Ministry of innovation, sciences and research of the State of Nordrhein-Westfalen

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Project 3:

Real-time Image Processing of Super-Resolution Optical Microscopy Data of Living Cells

Prof. Dr. Thomas Huser (biomolecular photonics, experiments), Bielefeld University
Prof. Dr. Marc-Oliver Schierenberg (image processing, spectroscopy), FH Bielefeld
Prof. Dr. Christian Schröder (parallel and distributed computing), FH Bielefeld

An electron microscope has a much higher resolving power than a light microscope but is also much more expensive. Furthermore, it requires special preparation of objects, which makes it impossible to observe living objects. Optical microscopes allow for observing living objects but do not yield the resolution necessary for objects such as nanoparticles, proteins or viruses. The technique of "super-resolving" microscopy helps overcome this limitation and enables observation of viruses interacting with living cells, for example.

This project aims at reconstructing "super-resolved" images from a number of lower-resolved images obtained through an optical microscope. The reconstruction is done in real time using a GPU-based workstation and delivers unprecedented detailedness in space and time. A software package is being developed which enables interaction of the microscope and the computer, implements and optimizes the image reconstruction and allows for viewing and further processing of the images.

Comparison of microscopy images: left image widefield microscopy, middle image super-resolved image, right image one of the 15 SIM-images used for the reconstruction of the middle image Screenshot der GUI zur Bildbearbeitung